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Part:BBa_K5423001:Design

Designed by: Kyle Cavanagh   Group: iGEM24_ANU   (2024-10-02)


KatG E. coli dCas9 gRNA


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 32
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 5
    Illegal BsaI.rc site found at 41


Design Notes

The gRNA was designed in such a way that it can only interact with the KatG gene, and no other genes.


Source

Generated in ChopChop

References